Resumen

BOHORQUEZ VARGAS, Joshua Alejandro et al. Effect of aqueous extract of Ocotea quixos leaf on Rhipicephalus microplus in vitro. Siembra [online]. 2026, vol.13, n.1, e8162. ISSN 2477-8850.  https://doi.org/10.29166/siembra.v13i1.8162.

Ticks seriously affect cattle in tropical areas, being Rhipicephalus microplus (Ixodida: Ixodidae) the most relevant species due to its ability to transmit diseases and generate significant economic losses. The objective of this study was to evaluate the tick-killing action of the aqueous extract of the leaf of Ocotea quixos against R. microplus. A completely randomized design with a factorial arrangement was employed, in which three concentrations of the hydrolate (25%, 62.5%, and 100%) and three immersion times (1.0, 3.5, and 6.0 minutes) were analyzed, along with positive and negative controls. The tests were conducted under controlled conditions, and mortality was measured every 4 hours for 7 days. The results showed that the hydrolate concentration and its interaction with exposure time significantly influenced tick mortality (p < 0.0001), highlighting the 100% treatment applied for 6 minutes, which achieved 87% mortality, a value comparable to the positive control (99.1%). The predictive model showed a high fit (R² = 0.9973), allowing the effects of the treatment to be accurately anticipated. It was verified that immersion time only improves efficacy at low concentrations. The aqueous extract of O. quixos represents an effective natural alternative to synthetic acaricides, contributing to the sustainability of livestock production through biological control strategies with a lower environmental impact.

Palabras clave : Amazon cinnamon; cinnamaldehyde; hidrosol; ishpingo; tick.

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