<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2528-7907</journal-id>
<journal-title><![CDATA[Revista San Gregorio]]></journal-title>
<abbrev-journal-title><![CDATA[Revista San Gregorio]]></abbrev-journal-title>
<issn>2528-7907</issn>
<publisher>
<publisher-name><![CDATA[Universidad San Gregorio de Portoviejo ]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2528-79072023000300092</article-id>
<article-id pub-id-type="doi">10.36097/rsan.v1i55.2525</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Evaluación de métodos de extracción proteica en alevines de chame (Dormitator latifrons) para estudio proteómico]]></article-title>
<article-title xml:lang="en"><![CDATA[Evaluation of protein extraction methods in fingerlings of chame (Dormitator latifrons) for proteomic study]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Zambrano Ostaiza]]></surname>
<given-names><![CDATA[Agustin Norberto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Espinosa López.]]></surname>
<given-names><![CDATA[Elisa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gómez Baena]]></surname>
<given-names><![CDATA[Guadalupe]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vera Delgado]]></surname>
<given-names><![CDATA[Juan Manuel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Técnica de Manabí  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Ecuador</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de Córdoba  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Spain</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad de Córdoba  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Spain</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Universidad Técnica de Manabí  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Ecuador</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>11</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>11</month>
<year>2023</year>
</pub-date>
<volume>1</volume>
<numero>55</numero>
<fpage>92</fpage>
<lpage>106</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_arttext&amp;pid=S2528-79072023000300092&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_abstract&amp;pid=S2528-79072023000300092&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_pdf&amp;pid=S2528-79072023000300092&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen El chame (Dormitator latifrons), es un pez de agua dulce, con potencial acuícola en Latinoamérica. Su producción, está limitada a la utilización de juveniles capturados del medio silvestre. Se ha logrado reproducir esta especie en cautiverio a partir de reproductores obtenidos del medio natural, aunque en la mayoría de los casos, el proceso fracasa en el paso de prolarvas a larvas por falta de alimento que contenga los requerimientos nutricionales necesarios, especialmente proteínas como unos de los principales compuestos que necesitan los peces. El objetivo de la investigación fue evaluar dos protocolos de extracción de proteínas, a partir de alevines de Dormitator latifrons. Se tomaron muestras de alevines del medio natural, se sacrificaron por inmersión en N2 líquido y conservados a -80°C hasta la extracción de proteínas. Este trabajo compara la concentración de proteína y el perfil proteico, obtenidos mediante el uso de dos tampones de extracción: uno tradicional RIPA; y bicarbonato amónico, una sal volátil compatible con espectrometría de masas. Como resultado se muestra un mejor perfil proteico en las muestras tratadas con bicarbonato amónico, por lo que se concluye y recomienda este protocolo para futuras investigaciones del proteoma global, mediante espectrometría de masas.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract The chame (Dormitator latifrons) is a freshwater fish with aquaculture potential in Latin America. Its production is limited to the use of juveniles captured from the wild. It has been possible to reproduce this species in captivity from broodstock obtained from the natural environment, although in most cases, the process fails in the passage from prolarvae to larvae due to lack of food that contains the necessary nutritional requirements, especially proteins such as some of the main compounds needed by fish. The objective of the research was to evaluate two protein extraction protocols from Dormitator latifrons fingerlings. Fingerling samples were taken from the natural environment, sacrificed by immersion in liquid N2 and stored at -80°C until protein extraction. This work compares the protein concentration and the protein profile, obtained by using two extraction buffers: a traditional RIPA; and ammonium bicarbonate, a volatile salt compatible with mass spectrometry. As a result, a better protein profile is shown in the samples treated with ammonium bicarbonate, which is why this protocol is concluded and recommended for future investigations of the global proteome, by means of mass spectrometry.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Dormitator latifrons]]></kwd>
<kwd lng="es"><![CDATA[alevines]]></kwd>
<kwd lng="es"><![CDATA[cuantificación]]></kwd>
<kwd lng="es"><![CDATA[perfil proteico]]></kwd>
<kwd lng="es"><![CDATA[proteómica]]></kwd>
<kwd lng="en"><![CDATA[Dormitator latifrons]]></kwd>
<kwd lng="en"><![CDATA[fingerlings]]></kwd>
<kwd lng="en"><![CDATA[quantification]]></kwd>
<kwd lng="en"><![CDATA[protein profile]]></kwd>
<kwd lng="en"><![CDATA[proteomics.]]></kwd>
</kwd-group>
</article-meta>
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