<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2477-9105</journal-id>
<journal-title><![CDATA[Perfiles]]></journal-title>
<abbrev-journal-title><![CDATA[Perfiles]]></abbrev-journal-title>
<issn>2477-9105</issn>
<publisher>
<publisher-name><![CDATA[Escuela Superior Politécnica de Chimborazo]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2477-91052019000100020</article-id>
<article-id pub-id-type="doi">10.47187/perf.v2i22.50</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[ESTUDIO DEL METABOLOMA EN EL ANTAGONISMO MICROBIANO A TRAVÉS DE CROMATOGRAFÍA LÍQUIDA CON ALGORITMOS QUIMIOMÉTRICOS]]></article-title>
<article-title xml:lang="en"><![CDATA[Metabolome analysis in the microbial antagonism by liquid chromatography coupled with chemometrics algorithms]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chalén-Alvarado]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Quiroz-Moreno]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mogollón]]></surname>
<given-names><![CDATA[NGS]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Domínguez]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Escuela Superior Politécnica del Litoral, ESPOL Centro Nacional de Acuicultura e Investigaciones Marinas - CENAIM ]]></institution>
<addr-line><![CDATA[Guayaquil ]]></addr-line>
<country>Ecuador</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Regional Amazónica Ikiam  ]]></institution>
<addr-line><![CDATA[Tena Napo]]></addr-line>
<country>Ecuador</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2019</year>
</pub-date>
<volume>2</volume>
<numero>22</numero>
<fpage>20</fpage>
<lpage>25</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_arttext&amp;pid=S2477-91052019000100020&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_abstract&amp;pid=S2477-91052019000100020&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_pdf&amp;pid=S2477-91052019000100020&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen El metaboloma es el conjunto de compuestos orgánicos de bajo peso molecular (metabolitos) producidos por sistemas biológicos. El antagonismo microbiano es una importante fuerza evolutiva, por lo que el análisis de su metaboloma es una herramienta útil para la búsqueda de nuevas moléculas con actividad biológica. El objetivo de este trabajo fue implementar el uso de algoritmos quimiométricos para la identificación de variaciones en el metaboloma del antagonismo microbiano entre Pseudovibrio denitrificans y Vibrio harveyi. Extractos de bacterias y del medio de cultivo usado fueron analizados por cromatografía líquida de ultra alta eficiencia acoplada a un detector de arreglo de diodos (UHPLC-DAD). Además, algoritmos quimiométricos fueron aplicados realizando un Análisis de Componentes Principales (PCA) de los cromatogramas obtenidos. Se encontraron tres picos que expresaron una mayor variabilidad entre el metaboloma individual y el metaboloma de la interacción de P. denitrificans y V. harveyi. De esta manera, la metabolómica con UHPLC-DAD y algoritmos quimiométricos demostró ser una herramienta útil para la identificación de picos responsables de las diferencias entre interacciones microbianas.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Metabolome is a group of low molecular weight organic compounds (metabolites) produced by biological systems. Bacterial antagonism is an important evolving force, in this sense analysis of its metabolome represents a useful tool for discovering new molecules with biological activity. The objective of this research work was to implement the use of chemometric algorithms for the identification of variations in the metabolome of the microbial antagonism between Pseudovibrio denitrificans and Vibrio harveyi. Extracts from cultured bacteria and used culture media were analyzed by Ultra-high performance liquid chromatography coupled with a diode-array detector (UHPLC-DAD). Additionally, chemometric algorithms were employed, subjecting to Principal Component Analysis (PCA) for the obtained chromatograms. Three peaks were found that express the major variability between the individual metabolome and the metabolome from the interaction of P. denitrificans and V. harveyi. In this manner, metabolomic through UHPLC-DAD and chemometric algorithms, showed to be a useful tool to identify the peaks responsible for differences between microbial interactions.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Antagonismo microbiano]]></kwd>
<kwd lng="es"><![CDATA[cromatografía líquida]]></kwd>
<kwd lng="es"><![CDATA[análisis de componentes principales]]></kwd>
<kwd lng="es"><![CDATA[Pseudovibrio denitrificans]]></kwd>
<kwd lng="es"><![CDATA[Vibrio harveyi]]></kwd>
<kwd lng="en"><![CDATA[Microbial antagonism]]></kwd>
<kwd lng="en"><![CDATA[liquid chromatography]]></kwd>
<kwd lng="en"><![CDATA[principal component analysis]]></kwd>
<kwd lng="en"><![CDATA[Pseudovibrio denitrificans]]></kwd>
<kwd lng="en"><![CDATA[Vibrio harveyi.]]></kwd>
</kwd-group>
</article-meta>
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