<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1390-8596</journal-id>
<journal-title><![CDATA[LA GRANJA. Revista de Ciencias de la Vida ]]></journal-title>
<abbrev-journal-title><![CDATA[La Granja]]></abbrev-journal-title>
<issn>1390-8596</issn>
<publisher>
<publisher-name><![CDATA[Universidad Politécnica Salesiana]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1390-85962022000200113</article-id>
<article-id pub-id-type="doi">10.17163/lgr.n36.2022.09</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Efecto de factores embriotróficos a diferentes tensiones de oxígeno en cultivo in vitro sobre el desarrollo embrionario de alpacas hasta la etapa de blastocisto]]></article-title>
<article-title xml:lang="en"><![CDATA[Effect of embryotrophic factors at different oxygen tension for in vitro culture on the embryonic development of alpacas up to the blastocyst stage]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Huanca Mamani]]></surname>
<given-names><![CDATA[Teodosio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Nacional de Innovación Agraria  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Perú</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>02</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>02</month>
<year>2022</year>
</pub-date>
<volume>36</volume>
<numero>2</numero>
<fpage>113</fpage>
<lpage>121</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_arttext&amp;pid=S1390-85962022000200113&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_abstract&amp;pid=S1390-85962022000200113&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_pdf&amp;pid=S1390-85962022000200113&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen La alpaca es el camélido sudamericano doméstico de mayor importancia para el Perú, país que a nivel regional cuenta con el 87% de estos animales. Ya que las formas tradicionales de reproducción no garantizan su calidad genética, la reproducción in vitro es una alternativa para su mejoramiento. Este estudio evaluó la influencia de los factores embriotróficos de desarrollo epidérmico (EGF) y de crecimiento insulínico (IGF-1) y tensiones de oxígeno en el desarrollo in vitro de ovocitos de alpaca hasta la etapa de blastocistos. A partir de ovarios de animales sacrificados, se obtuvieron ovocitos que se colocaron en medio TCM-199, suplementado con piruvato de sodio, glutamina, estradiol (E2), hormona folículo estimulante (FSH), hormona luteinizante (LH), factor embriotrófico (EGF o IGF-1), 5% de suero fetal bovino y gentamicina (10.L/mL) durante 32 horas, a 38,5 .C, con 5% de CO. y humedad relativa mayor de 95%, con tensiones O. entre 6 y 20%. Posteriormente, los ovocitos fueron fecundados con semen fresco y cultivados en medio KSOMaa durante 48 horas. Los cultivos fueron diferenciados por factores de crecimiento (EGF e IGF-1) y tensiones de O. (6% y 20%), más el grupo control sin suplementar con EGF o IGF-I, para valorar las tasas de división de ovocitos y blastocistos a partir de ovocitos. Mediante análisis de conglomerados, se establecieron diferencias significativas entre los tratamientos con &#945;= 0,05 para cada variable de respuesta, observándose la mayor tasa de divisiones de ovocitos (24,8%) con EGF a 6% de O.y la mayor producción de blastocistos/ovocito (18,4%) con IGF-1 a 6% de O. . Se concluye que la adición de factores embriotróficos y una baja tensión de O. son favorables para el desarrollo embrionario in vitro en alpacas.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract The alpaca is the most important South American domestic camelid for Peru, a country that regionally has 87% of these animals. Since traditional forms of reproduction do not guarantee its genetic quality, in vitro reproduction is an alternative for its improvement. This study evaluated the influence of embryotrophic factors of epidermal development (EGF) and insulin-like growth factor (IGF-1) and oxygen tension on the in vitro development of alpaca oocytes up to the blastocyst stage. From ovaries of sacrificed animals, oocytes were obtained and placed in TCM-199 medium, supplemented with sodium pyruvate, glutamine, estradiol (E2), follicle stimulating hormone (FSH), luteinizing hormone (LH), embryotrophic factor (EGF or IGF-1), 5% fetal bovine serum and gentamicin (10.L/mL) for 32 hours, at 38.5 .C, with 5% CO. and relative humidity greater than 95%, with O. tensions between 6 and 20%. Subsequently, the oocytes were fertilized with fresh semen and cultured in KSOMaa medium for 48 hours. Cultures were differentiated by growth factors (EGF and IGF-1) and O. tensions (6% and 20%), plus the control group without EGF or IGF-I supplementation, to assess oocyte and blastocyst division rates from oocytes. By cluster analysis, significant differences were established between treatments with &#945;= 0.05 for each response variable, with the highest rate of oocyte divisions (24.8%) with EGF at 6% O. and the highest blastocyst/oocyte production (18.4%) with IGF-1 at 6% O.. It is concluded that the addition of embryotrophic factors and low O. tension are favorable for in vitro embryo development in alpacas.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Alpaca]]></kwd>
<kwd lng="es"><![CDATA[camélidos]]></kwd>
<kwd lng="es"><![CDATA[clivaje]]></kwd>
<kwd lng="es"><![CDATA[fertilización]]></kwd>
<kwd lng="es"><![CDATA[ovocitos]]></kwd>
<kwd lng="es"><![CDATA[reproducción]]></kwd>
<kwd lng="en"><![CDATA[Alpaca]]></kwd>
<kwd lng="en"><![CDATA[camelids]]></kwd>
<kwd lng="en"><![CDATA[cleavage]]></kwd>
<kwd lng="en"><![CDATA[fertilization]]></kwd>
<kwd lng="en"><![CDATA[oocytes]]></kwd>
<kwd lng="en"><![CDATA[reproduction]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Comparative patterns of follicle development and selection in ruminants]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Adams,]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<source><![CDATA[Journal of Reproduction and Fertility-Supplement]]></source>
<year>1999</year>
</nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ahumada,]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
</person-group>
<source><![CDATA[Efecto de factores de crecimiento en el cultivo sobre el desarrollo y calidad de embriones bovinos producidos in vitro en grupos reducidos]]></source>
<year>2011</year>
</nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Efecto de la suplementación con alanina y glicina sobre los clivajes iniciales de embiones bovinos producidos in vitro]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Arias,]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
</person-group>
<source><![CDATA[Revista MVZ Córdoba]]></source>
<year>2007</year>
</nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Efecto del Método de Colección y Tensión de Oxígeno sobre el Desarrollo de Ovocitos Bovinos Fecundados y Cultivados in vitro]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Benavides,]]></surname>
<given-names><![CDATA[L.,]]></given-names>
</name>
<name>
<surname><![CDATA[Huanca]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
<name>
<surname><![CDATA[Quintanilla]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<source><![CDATA[Revista de Investigaciones Veterinarias del Perú]]></source>
<year>2015</year>
</nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Use of insulin-like growth factor1 to improve post-transfer survival of bovine embryos produced in vitro]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Block,]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<source><![CDATA[Theriogenology]]></source>
<year>2007</year>
</nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Mathematical modelling of oxygen concentration in bovine and murine cumulus&#8211;oocyte complexes]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Clark,]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<source><![CDATA[Reproduction]]></source>
<year>2006</year>
</nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Cuadras,]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
</person-group>
<source><![CDATA[Nuevos métodos de análisis multivariante]]></source>
<year>2020</year>
</nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Efecto de tres niveles de oxígeno en la atmósfera de cultivo y la adición de un antioxidante comercial en el desarrollo de embriones bovinos producidos in vitro]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Delgado,]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<source><![CDATA[Acta universitaria]]></source>
<year>2018</year>
</nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Díaz,]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<source><![CDATA[Estudio de caracterización climática de la precipitación pluvial y temperatura del aire para la cuenca de los ríos Coata e Ilave]]></source>
<year>2013</year>
</nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Importance of lipid metabolism on oocyte maturation and early embryo development: Can we apply what we know to buffalo?]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Dubeibe,]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<source><![CDATA[Animal reproduction science]]></source>
<year>2019</year>
</nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Generalidades de la producción de embriones bovinos in vitro]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gonella Diaza,]]></surname>
<given-names><![CDATA[Á.]]></given-names>
</name>
</person-group>
<source><![CDATA[Revista de Investigación Agraria y Ambiental]]></source>
<year>2013</year>
</nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Bovine blastocyst development after in vitro maturation in a defined medium with epidermal growth factor and low concentrations of gonadotropins]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Harper,]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Brackett]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
</person-group>
<source><![CDATA[Biology of reproduction]]></source>
<year>1993</year>
</nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Low oxygen tension during in vitro maturation is beneficial for supporting the subsequent development of bovine cumulus&#8211;oocyte complexes]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hashimoto,]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular Reproduction and Development: Incorporating Gamete Research]]></source>
<year>2000</year>
</nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Low oxygen concentrations improve yak oocyte maturation and enhance the developmental competence of preimplantation embryos]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[He,]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<source><![CDATA[Theriogenology]]></source>
<year>2020</year>
</nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Huanca,]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<source><![CDATA[XVI Congreso Venezolano de Producción e industria Animal, Asociación Venezolana de Producción Animal]]></source>
<year>2012</year>
</nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="">
<collab>INEI</collab>
<source><![CDATA[Resultados definitivos del IV censo nacional agropecuario &#8211; 2012]]></source>
<year>2013</year>
</nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Low oxygen tension during in vitro maturation of porcine follicular oocytes improves parthenogenetic activation and subsequent development to the blastocyst stage]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Iwamoto,]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Theriogenology]]></source>
<year>2005</year>
</nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[IGF-1 treatment during in vitro maturation improves developmental potential of ovine oocytes through the regulation of PI3K/Akt and apoptosis signaling]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Javvaji,]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<source><![CDATA[Animal biotechnology]]></source>
<year>2020</year>
</nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Peptide growth factors and preimplantation development]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Kane,]]></surname>
<given-names><![CDATA[M.,]]></given-names>
</name>
<name>
<surname><![CDATA[Morgan]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Coonan]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
</person-group>
<source><![CDATA[Human reproduction update]]></source>
<year>1997</year>
</nlm-citation>
</ref>
<ref id="B20">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Free radical scavengers ameliorate the 2-cell block in mouse embryo culture]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Legge,]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Sellens]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Human Reproduction]]></source>
<year>1991</year>
</nlm-citation>
</ref>
<ref id="B21">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Papel del factor de crecimiento semejante a insulin-1 (IGF-1) en la regulación de la función ovárica]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Lenz,]]></surname>
<given-names><![CDATA[M.,]]></given-names>
</name>
<name>
<surname><![CDATA[Benavides]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Uribe-Velásquez]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<source><![CDATA[Biosalud]]></source>
<year>2007</year>
</nlm-citation>
</ref>
<ref id="B22">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Effects of L-carnitine on embryo development of vitrified swamp buffalo oocytes following in vitro fertilization]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Liang,]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
</person-group>
<source><![CDATA[Livestock Science]]></source>
<year>2020</year>
</nlm-citation>
</ref>
<ref id="B23">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Effect of retinoids and growth factor on in vitro bovine embryos produced under chemically defined conditions]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Lima,]]></surname>
<given-names><![CDATA[PF]]></given-names>
</name>
</person-group>
<source><![CDATA[Animal reproduction science]]></source>
<year>2006</year>
</nlm-citation>
</ref>
<ref id="B24">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Modulación del estado de óxido-reducción por peróxido de hidrógeno en la etapa de maduración ovocitaria: efecto sobre el desarrollo embrionario en bovinos]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Loren,]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<source><![CDATA[International Journal of Morphology]]></source>
<year>2016</year>
</nlm-citation>
</ref>
<ref id="B25">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Comparación de dos técnicas para la obtención de complejos cumulus ovocito porcinos]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Lorenzo,]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[InVet]]></source>
<year>2015</year>
</nlm-citation>
</ref>
<ref id="B26">
<nlm-citation citation-type="">
<collab>MINAGRI</collab>
<source><![CDATA[Población y producción animal]]></source>
<year>2015</year>
</nlm-citation>
</ref>
<ref id="B27">
<nlm-citation citation-type="">
<collab>MINAGRI</collab>
<source><![CDATA[Potencial productivo y comercial de la alpaca]]></source>
<year>2019</year>
</nlm-citation>
</ref>
<ref id="B28">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Involvement of superoxide radicals in the mouse two-cell block]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Noda,]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular reproduction and development]]></source>
<year>1991</year>
</nlm-citation>
</ref>
<ref id="B29">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Evaluación de la eficiencia de la transferencia de embriones Inter especie entre alpacas y llamas obtenidos por ovulación simple]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pacheco,]]></surname>
<given-names><![CDATA[J.,]]></given-names>
</name>
<name>
<surname><![CDATA[Vélez]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
<name>
<surname><![CDATA[Pezo]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<source><![CDATA[Revista de Investigaciones Veterinarias del Perú]]></source>
<year>2016</year>
</nlm-citation>
</ref>
<ref id="B30">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Comparación de sistemas de cultivo de embriones de alpacas]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pérez,]]></surname>
<given-names><![CDATA[M.,]]></given-names>
</name>
<name>
<surname><![CDATA[Zevallos]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Perez]]></surname>
<given-names><![CDATA[U.]]></given-names>
</name>
</person-group>
<source><![CDATA[Revista de Investigaciones Altoandinas]]></source>
<year>2017</year>
</nlm-citation>
</ref>
<ref id="B31">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[The epidermal growth factor network: role in oocyte growth, maturation and developmental competence]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Richani,]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Gilchrist]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<source><![CDATA[Human reproduction update]]></source>
<year>2018</year>
</nlm-citation>
</ref>
<ref id="B32">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Similar patterns of embryo development in canine oocytes cultured in vitro at oxygen tensions of 5 and 20%]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rodrigues,]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
</person-group>
<source><![CDATA[Theriogenology]]></source>
<year>2013</year>
</nlm-citation>
</ref>
<ref id="B33">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[In vitro production and transfer of embryos in South American Camelids: New opportunities and challenge]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ruiz,]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
</person-group>
<source><![CDATA[Spermova]]></source>
<year>2018</year>
</nlm-citation>
</ref>
<ref id="B34">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Efecto del factor de crecimiento epidermal (EGF) sobre la maduración de ovocitos bovinos cultivados In vitro]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Salgado,]]></surname>
<given-names><![CDATA[R.,]]></given-names>
</name>
<name>
<surname><![CDATA[Simanca]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Vergara]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
</person-group>
<source><![CDATA[Revista Científica]]></source>
<year>2013</year>
</nlm-citation>
</ref>
<ref id="B35">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[TUNEL analyses of bovine blastocysts after culture with EGF and IGF-I]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sirisathien,]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Brackett]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular Reproduction and Development: Incorporating Gamete Research]]></source>
<year>2003</year>
</nlm-citation>
</ref>
<ref id="B36">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Desarrollo embrionario de bovino in vitro cocultivado con células oviductales y del cumulus oophorus]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Soto-Martínez,]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
</person-group>
<source><![CDATA[Revista de Salud Animal]]></source>
<year>2019</year>
</nlm-citation>
</ref>
<ref id="B37">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Effect of angiotensin II with follicle cells and insulin-like growth factorI or insulin on bovine oocyte maturation and embryo development]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Stefanello,]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<source><![CDATA[Theriogenology]]></source>
<year>2006</year>
</nlm-citation>
</ref>
<ref id="B38">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Effects of oxygen toxicity on early development of mouse embryos]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Umaoka,]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular reproduction and development]]></source>
<year>1992</year>
</nlm-citation>
</ref>
<ref id="B39">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[The developmental potential of the human oocyte is related to the dissolved oxygen content of follicular fluid: association with vascular endothelial growth factor levels and perifollicular blood flow characteristics]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Van Blerkom,]]></surname>
<given-names><![CDATA[J.,]]></given-names>
</name>
<name>
<surname><![CDATA[Antczak]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Schrader]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<source><![CDATA[Human reproduction]]></source>
<year>1997</year>
</nlm-citation>
</ref>
<ref id="B40">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Kaempferol attenuates mitochondrial dysfunction and oxidative stress indu ced by H2O2 during porcine embryonic development]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Yao,]]></surname>
<given-names><![CDATA[X.]]></given-names>
</name>
</person-group>
<source><![CDATA[Theriogenology]]></source>
<year>2019</year>
</nlm-citation>
</ref>
<ref id="B41">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[reatment of epidermal growth factor (EGF) enhances nuclear maturation of porcine oocytes and stimulates expression of ER/Golgi transport proteins]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Yong,]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<source><![CDATA[Development &amp; reproduction]]></source>
<year>2017</year>
</nlm-citation>
</ref>
</ref-list>
</back>
</article>
