<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2602-8492</journal-id>
<journal-title><![CDATA[Revista Técnica energía]]></journal-title>
<abbrev-journal-title><![CDATA[Revista Técnica energía]]></abbrev-journal-title>
<issn>2602-8492</issn>
<publisher>
<publisher-name><![CDATA[Operador Nacional de Electricidad CENACE]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2602-84922020000100126</article-id>
<article-id pub-id-type="doi">10.37116/revistaenergia.v16.n2.2020.360</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Optimización de la Producción de Xilanasa Bacteriana a Partir de Bacillus sp. K1 con el Uso de Residuos Lignocelulósicos]]></article-title>
<article-title xml:lang="en"><![CDATA[Optimization of Xylanase Production by Bacillus sp. K1 Using Lignocellulo-sic Residues]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Solis]]></surname>
<given-names><![CDATA[Terry]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Calderón]]></surname>
<given-names><![CDATA[Cristina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Yang]]></surname>
<given-names><![CDATA[Liu]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Escuela Superior Politécnica de Chimborazo  ]]></institution>
<addr-line><![CDATA[Riobamba ]]></addr-line>
<country>Ecuador</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Escuela Superior Politécnica de Chimborazo  ]]></institution>
<addr-line><![CDATA[Riobamba ]]></addr-line>
<country>Ecuador</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Huzhou University, Huzhou Escuela de Ciencias de la Vida ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>China</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2020</year>
</pub-date>
<volume>16</volume>
<numero>2</numero>
<fpage>126</fpage>
<lpage>134</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_arttext&amp;pid=S2602-84922020000100126&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_abstract&amp;pid=S2602-84922020000100126&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://scielo.senescyt.gob.ec/scielo.php?script=sci_pdf&amp;pid=S2602-84922020000100126&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: Las xilanasas son enzimas que hidrolizan el xilano, produciendo azúcares fermentables tales como xilosa y xilobiosa con aplicaciones industriales muy diversas entre las que destaca el biobleaching y la producción de bioetanol de segunda generación. El objetivo principal del presente estudio se enfoca en la optimización de la producción de xilanasa bacteriana por parte de la bacteria Bacillus sp. K1. La misma ha sido previamente aislada de muestras de madera en putrefacción recolectadas en las premisas de la Universidad de Lakehead para su Departamento de Biología. Las variables investigadas para la optimización fueron físicas tales como pH, temperatura y volumen de inóculo; y de composición del medio de fermentación tales como fuente de carbono, fuente de nitrógeno orgánico e inorgánico y relación porcentual entre dichos componentes. Adicionalmente, se determinó el efecto de iones metálicos (sodio, potasio, magnesio, calcio, ferroso, niqueloso, cúprico, cobaltoso y manganoso) y surfactantes (Polisorbato-20, dodecil sulfato de sodio y Triton X-100) en la actividad enzimática de la xilanasa producida. La mayor producción de xilanasa fue tras 36h de fermentación y las condiciones físicas óptimas asociadas a la producción de xilanasa fueron pH 6, temperatura 35 ºC y volumen de inóculo 1%. La composición óptima del medio de fermentación determinada está constituida por: Afrecho de trigo 4%, Glucosa 0,5%, NH4NO3 0,5%, K2HPO4 0,1%, KCl 0,1%, MgSO4 0,05%, peptona 0,5%, SDS 0,1%. La actividad enzimática generada es de 264,96±7,89 IU/L, con un 248,79% de incremento con respecto al inicial. Los inhibidores más importantes fueron los iones cúprico y manganoso con 32,00% y 49,16% de inhibición comparado con el experimento de control.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: The xylanases are enzymes that hydrolyze xylan, producing fermentable sugars such as xylose and xylobiose. These enzymes have several industrial applications, among which its use in biobleaching and second-generation bioethanol stands out. The main objective in the present study is the optimization of the bacterial xylanase production by the strain Bacillus sp. K1, which has been previously isolated from rotting wood samples collected at Lakehead University's premises for its Biology Department. The variables investigated for optimization were physical such as pH, temperature and inoculum volume; and composition of the fermentation medium such as carbon source, organic and inorganic nitrogen source and percentage ratio between these components. Additionally, the effect of metal ions (sodium, potassium, magnesium, calcium, ferrous, nickelous, cupric, cobaltous and manganous) and surfactants (Tween-20, dodecyl sodium sulfate and Triton X-100) in the xylanase enzyme activity was determined. The highest production of xylanase was obtained after 36h fermentation and the optimal physical conditions associated with the xylanase production were pH 6, temperature 35 ºC and inoculum volume 1%. The optimum composition of the fermentation medium consists of: wheat bran 4%, glucose 0,5%, NH4NO3 0,5%, K2HPO4 0,1%, KCl 0,1%, MgSO4 0,05%, peptone 0,5%, SDS 0,1%. The enzyme activity generated is 264,96±7,89 IU/L, with a 248,79% increase from the initial conditions. The most important inhibitors were cupric and manganous ions with 32,00% and 49,16% inhibition compared to the control experiment.]]></p></abstract>
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<kwd lng="es"><![CDATA[Acreditación]]></kwd>
<kwd lng="es"><![CDATA[Laboratorio]]></kwd>
<kwd lng="es"><![CDATA[Ensayos Estructurales]]></kwd>
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<kwd lng="en"><![CDATA[Rollover]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[The path forward for biofuels and biomaterials]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ragauskas]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Williams]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Davison]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
<name>
<surname><![CDATA[Britovsek]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Cairney]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Eckert]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Frederick]]></surname>
<given-names><![CDATA[W. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Hallett]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<given-names><![CDATA[D. Leak]]></given-names>
</name>
<name>
<surname><![CDATA[Liotta]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Mielenz]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Murphy]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Templer]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Tschaplinski]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<source><![CDATA[Science]]></source>
<year>2006</year>
<volume>311</volume>
<page-range>484-9</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Molecular approaches for ameliorating microbial xylanases]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Verma]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Satyanarayana]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<source><![CDATA[Bioresource Technology]]></source>
<year>2012</year>
<page-range>360-7</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Xylanases, xylanase families and extremophilic xylanases]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Collins]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Gerday]]></surname>
<given-names><![CDATA[C.]]></given-names>
</name>
<name>
<surname><![CDATA[Feller]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<source><![CDATA[FEMS Microbiology Reviews]]></source>
<year>2015</year>
<page-range>3-23</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Isolation and characterization of a xylanase from Bacillus subtilis.]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bernier]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Desrochers]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Jurasek]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Paice]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Applied and Environmental Microbiology]]></source>
<year>1983</year>
<page-range>511-4</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Multiplicity of &#946;-1,4-xylanase in microorganisms:functions and applications]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wong]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
<name>
<surname><![CDATA[Tan]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Saddler]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<source><![CDATA[Microbiological Reviews]]></source>
<year>1988</year>
<page-range>305-17</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Biotechnology of microbial xylanases: enzymology, molecular biology, and application]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Subramaniyan]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Prema]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<source><![CDATA[Critical Reviews in Biotechnology]]></source>
<year>2002</year>
<page-range>33-64</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Pretreatment of Lignocellulosic Wastes to Improve Ethanol and Biogas Production: A Review]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Taherzadeh]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Karimi]]></surname>
<given-names><![CDATA[K.]]></given-names>
</name>
</person-group>
<source><![CDATA[International Journal of Molecular Sciences]]></source>
<year>2008</year>
<page-range>707-38</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Solis]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<source><![CDATA[Optimización de la producción de xilanasa bacteriana a partir de Bacillus sp. K1 con el uso de residuos lignocelulósicos en el Departamento de Biología de la Universidad de Lakehead, Canadá]]></source>
<year></year>
</nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Characterization of Novel Cellulase-producing Bacteria]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Prasad]]></surname>
<given-names><![CDATA[Y.]]></given-names>
</name>
<name>
<surname><![CDATA[Qin]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<source><![CDATA[Applied Biochemistry and Biotechnology]]></source>
<year>2015</year>
<volume>177</volume>
<page-range>1186-98</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Analyzing Differences in Bacterial Optical Density Measurements]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Matlock]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
<name>
<surname><![CDATA[Beringer]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Ash]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Allen]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Page]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<source><![CDATA[Thermo Scientific]]></source>
<year>2007</year>
<page-range>1-2</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="">
<collab>Biotek Instruments</collab>
<source><![CDATA[]]></source>
<year></year>
</nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Use of dinitrosalicylic acid reagent for determination]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Miller]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<source><![CDATA[Analytical Chemistry]]></source>
<year>1959</year>
<page-range>426-8</page-range></nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Enzymes produced by biomass-degrading bacteria can ef&#64257;ciently]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Guo]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Chen]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Fan]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Zheng]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
<name>
<surname><![CDATA[Jian]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Qin]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<source><![CDATA[Renewable Energy]]></source>
<year>2017</year>
<page-range>195-201</page-range></nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Optimization of process parameters for xylanase production by Bacillus sp. in submerged fermentation]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Irfan]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Asghar]]></surname>
<given-names><![CDATA[U.]]></given-names>
</name>
<name>
<surname><![CDATA[Nadeem]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Nelofer]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Syed]]></surname>
<given-names><![CDATA[Q.]]></given-names>
</name>
</person-group>
<source><![CDATA[Journal of Radiation Research and Applied Sciences]]></source>
<year>2016</year>
<page-range>139-47</page-range></nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sharma]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Adhikari]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Satyanarayana]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<source><![CDATA[World J Microbiol Biotechnol]]></source>
<year>2007</year>
<page-range>483-90</page-range></nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Enhanced production of cellulase-free thermostable xylanase by Bacillus pumilus ASH and its potential application in paper industry]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Battan]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
<name>
<surname><![CDATA[Sharma]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
<name>
<surname><![CDATA[Dhiman]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Chander]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<source><![CDATA[Enzyme and Microbial Technology]]></source>
<year>2007</year>
<page-range>733-9</page-range></nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Optimization of xylanase production by Streptomyces sp P12-137 using response surface methodology and central composite design]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Coman]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
<name>
<surname><![CDATA[Bahrim]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<source><![CDATA[Annals of Microbiology]]></source>
<year>2011</year>
<page-range>773-9</page-range></nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Optimization of xylanase production by a hyperxylanolytic mutant strain of Fusarium oxysporum]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Chander]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Manchanda]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Singh]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<source><![CDATA[Process Biochemistry]]></source>
<year>1998</year>
<page-range>641-7</page-range></nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Production and optimization of thermostable alkaline xylanase by Penicillium oxalicum in solid state fermentation]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Muthezhilan]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Ashok]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<name>
<surname><![CDATA[Jayalakshmi]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<source><![CDATA[African Journal of Microbiology Research]]></source>
<year>2007</year>
<page-range>20-8</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
