Abstract

JARRIN RIOS, María Alejandra; CAICEDO BREEDDY, Maria Fernanda; GARRIDO VILLAVICENCIO, Pablo Ruben  and  CEPEDA INCA, Héctor Eduardo. Terapia fotodinámica antimicrobiana sobre Candida albicans en superficies acrílicas de prótesis dentales. Estudio in vitro. Rev Eug Esp [online]. 2022, vol.16, n.3, pp.72-82. ISSN 2661-6742.  https://doi.org/10.37135/ee.04.15.08.

Species of the genus Candida are frequently found in the oral microbiota of humans. Objective: to compare the antimicrobial effectiveness of photodynamic therapy on Candida albicans strains on acrylic surfaces for dental prostheses by using a 660nm wavelength laser and methylene blue as a photosensitizing agent, with respect to other therapeutic methods. Methodology: in vitro study, using 60 thermosetting acrylic discs immersed in a suspension of C. albicans, generating a biofilm simulation on the surface of a dental prosthesis. After that, five random groups of 12 units were formed to apply them different therapeutic procedures: G1 (saline solution), G2 (chlorhexidine 0.12%), G3 (nystatin in topical solution at 0.001ml/ul), G4 (0.005% methylene blue + diode laser with λ=660nm, 100mW, 32J/cm.) and G5 (0.01% methylene blue + diode laser with λ=660nm, 100mW, 321J/cm.). Results: a statistically significant difference was observed when comparing groups G4 and G5 with respect to G1 and G3 (.<0.05). Conclusion: the efficacy in reducing the number of viable C. albicans CFU was higher in the groups where laser radiation with a wavelength of 660nm was used with different concentrations of methylene blue. The use of nystatin and physiological saline had the lowest efficacy values.

Keywords : Photochemotherapy; Laser Therapy; Candidiasis, Oral; Dental Prosthesis.

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