Abstract

HUANCA MAMANI, Teodosio. Effect of embryotrophic factors at different oxygen tension for in vitro culture on the embryonic development of alpacas up to the blastocyst stage. La Granja [online]. 2022, vol.36, n.2, pp.113-121. ISSN 1390-8596.  https://doi.org/10.17163/lgr.n36.2022.09.

The alpaca is the most important South American domestic camelid for Peru, a country that regionally has 87% of these animals. Since traditional forms of reproduction do not guarantee its genetic quality, in vitro reproduction is an alternative for its improvement. This study evaluated the influence of embryotrophic factors of epidermal development (EGF) and insulin-like growth factor (IGF-1) and oxygen tension on the in vitro development of alpaca oocytes up to the blastocyst stage. From ovaries of sacrificed animals, oocytes were obtained and placed in TCM-199 medium, supplemented with sodium pyruvate, glutamine, estradiol (E2), follicle stimulating hormone (FSH), luteinizing hormone (LH), embryotrophic factor (EGF or IGF-1), 5% fetal bovine serum and gentamicin (10.L/mL) for 32 hours, at 38.5 .C, with 5% CO. and relative humidity greater than 95%, with O. tensions between 6 and 20%. Subsequently, the oocytes were fertilized with fresh semen and cultured in KSOMaa medium for 48 hours. Cultures were differentiated by growth factors (EGF and IGF-1) and O. tensions (6% and 20%), plus the control group without EGF or IGF-I supplementation, to assess oocyte and blastocyst division rates from oocytes. By cluster analysis, significant differences were established between treatments with α= 0.05 for each response variable, with the highest rate of oocyte divisions (24.8%) with EGF at 6% O. and the highest blastocyst/oocyte production (18.4%) with IGF-1 at 6% O.. It is concluded that the addition of embryotrophic factors and low O. tension are favorable for in vitro embryo development in alpacas.

Keywords : Alpaca; camelids; cleavage; fertilization; oocytes; reproduction.

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