Resumen

RAMIREZ CANDO, Lenin J.; GUERRA GUEVARA, Santiago P.  y  REINOSO MOLINA, Gabriela A.. In vitro evaluation of lead removal in wastewater by Photobacterium damselae. La Granja [online]. 2017, vol.26, n.2, pp.155-162. ISSN 1390-8596.  https://doi.org/10.17163/lgr.n26.2017.13.

In order to mitigate the environmental impacts caused by lead in wastewater from sectors such as: mining, petrochemical, metallurgical and others, an in vitro evaluation of lead removal using Photobacterium damselae was carried out. Considering isolation and biostimulation phase, obtained isolate was subjected to a selection process in a modified culture medium, to which concentrations of 20 and 100 ppm of Pb were added, finally obtaining the pure strain that showed resistance and /or tolerance to Pb. To determinate the remotion´s capacity of Pb in wastewater two conditions were observed: incubation at controlled temperature (25˚C) and incubation at room temperature in Quito-Ecuador (southern zone at 2800msnm). Biochemical characterization of the bacteria was performed using the GN-ID A + B Microgen Kit. In the development of bacterial growth kinetics curves and Pb removal curves, turbidimetry and atomic absorption techniques were used, it was noted that Photobacterium damselae presented a greater rate of growth to a maximum of 72 hours and a concentration of 20 ppm in incubation at room temperature achieving a removal rate of 69% of the lead in the medium. From this information, the potential of this bacterium is inferred and opportunities are opened to continue studies in the future.

Palabras clave : Photobacterium damselae; removal; bioremediation; lead; wastewaters.

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