SOTO VALENZUELA, Javier.; AMANO, Y.    DIAZ, L.. Evidence of rhizobium in Leucaena leucocephala nodules (Lam.), Using physicochemical culture techniques and scanning electron microscopy (SEM). []. , 4, 3, pp.133-138. ISSN 1390-7697.  https://doi.org/10.26423/rctu.v4i3.292.

Leucaena leucocephala L., is a Fabaceae with multipurpose agroforestry potential in Asia and Africa, while in Mesoamerica, where it originates, it is usually treated as a weed for cutting firewood and charcoal. Their study establishes that they are excellent producers of biomass in times of drought and how all legumes enter into symbiosis with their rhizobial hosts to fix nitrogen in the soil. In order to identify and characterize the presence of endophytic bacteria within L. leucocephala nodules, yeast, Mannitol, Congo Red Agar (LMRC) culture techniques, Gram stain tests, catalase, growth rate were used. colony morphology, acid production and growth in sodium chloride of isolates; complemented with scanning electron microscopy (SEM) techniques. The presence of bacteria inside the nodular roots

and in vitro culture was evidenced, collected in the practice campuses of the Península de Santa Elena State University (Santa Elena province, Ecuador). All the bacteria presented phenotypic and biochemical characteristics assigned to the group of rhizobia in their interaction with a wild legume little studied and exploited in the area.

: Rhizobia; Leucaena leucocephala; Scanning Electron Microscopy (SEM); Gram negative.

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